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Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease

BACKGROUND: The ability of circulating monocytes to develop into lung macrophages and promote lung tissue damage depends upon their phenotypic pattern of differentiation and activation. Whether this phenotypic pattern varies with COPD severity is unknown. Here we characterize the activation and diff...

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Autores principales: Cornwell, William D., Kim, Victor, Fan, Xiaoxuan, Vega, Marie Elena, Ramsey, Frederick V., Criner, Gerard J., Rogers, Thomas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003040/
https://www.ncbi.nlm.nih.gov/pubmed/29907106
http://dx.doi.org/10.1186/s12890-018-0664-y
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author Cornwell, William D.
Kim, Victor
Fan, Xiaoxuan
Vega, Marie Elena
Ramsey, Frederick V.
Criner, Gerard J.
Rogers, Thomas J.
author_facet Cornwell, William D.
Kim, Victor
Fan, Xiaoxuan
Vega, Marie Elena
Ramsey, Frederick V.
Criner, Gerard J.
Rogers, Thomas J.
author_sort Cornwell, William D.
collection PubMed
description BACKGROUND: The ability of circulating monocytes to develop into lung macrophages and promote lung tissue damage depends upon their phenotypic pattern of differentiation and activation. Whether this phenotypic pattern varies with COPD severity is unknown. Here we characterize the activation and differentiation status of circulating monocytes in patients with moderate vs. severe COPD. METHODS: Blood monocytes were isolated from normal non-smokers (14), current smokers (13), patients with moderate (9), and severe COPD (11). These cells were subjected to analysis by flow cytometry to characterize the expression of activation markers, chemoattractant receptors, and surface markers characteristic of either M1- or M2-type macrophages. RESULTS: Patients with severe COPD had increased numbers of total circulating monocytes and non-classical patrolling monocytes, compared to normal subjects and patients with moderate COPD. In addition, while the percentage of circulating monocytes that expressed an M2-like phenotype was reduced in patients with either moderate or severe disease, the levels of expression of M2 markers on this subpopulation of monocytes in severe COPD was significantly elevated. This was particularly evident for the expression of the chemoattractant receptor CCR5. CONCLUSIONS: Blood monocytes in severe COPD patients undergo unexpected pre-differentiation that is largely characteristic of M2-macrophage polarization, leading to the emergence of an unusual M2-like monocyte population with very high levels of CCR5. These results show that circulating monocytes in patients with severe COPD possess a cellular phenotype which may permit greater mobilization to the lung, with a pre-existing bias toward a potentially destructive inflammatory phenotype. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12890-018-0664-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-60030402018-07-06 Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease Cornwell, William D. Kim, Victor Fan, Xiaoxuan Vega, Marie Elena Ramsey, Frederick V. Criner, Gerard J. Rogers, Thomas J. BMC Pulm Med Research Article BACKGROUND: The ability of circulating monocytes to develop into lung macrophages and promote lung tissue damage depends upon their phenotypic pattern of differentiation and activation. Whether this phenotypic pattern varies with COPD severity is unknown. Here we characterize the activation and differentiation status of circulating monocytes in patients with moderate vs. severe COPD. METHODS: Blood monocytes were isolated from normal non-smokers (14), current smokers (13), patients with moderate (9), and severe COPD (11). These cells were subjected to analysis by flow cytometry to characterize the expression of activation markers, chemoattractant receptors, and surface markers characteristic of either M1- or M2-type macrophages. RESULTS: Patients with severe COPD had increased numbers of total circulating monocytes and non-classical patrolling monocytes, compared to normal subjects and patients with moderate COPD. In addition, while the percentage of circulating monocytes that expressed an M2-like phenotype was reduced in patients with either moderate or severe disease, the levels of expression of M2 markers on this subpopulation of monocytes in severe COPD was significantly elevated. This was particularly evident for the expression of the chemoattractant receptor CCR5. CONCLUSIONS: Blood monocytes in severe COPD patients undergo unexpected pre-differentiation that is largely characteristic of M2-macrophage polarization, leading to the emergence of an unusual M2-like monocyte population with very high levels of CCR5. These results show that circulating monocytes in patients with severe COPD possess a cellular phenotype which may permit greater mobilization to the lung, with a pre-existing bias toward a potentially destructive inflammatory phenotype. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12890-018-0664-y) contains supplementary material, which is available to authorized users. BioMed Central 2018-06-15 /pmc/articles/PMC6003040/ /pubmed/29907106 http://dx.doi.org/10.1186/s12890-018-0664-y Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cornwell, William D.
Kim, Victor
Fan, Xiaoxuan
Vega, Marie Elena
Ramsey, Frederick V.
Criner, Gerard J.
Rogers, Thomas J.
Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
title Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
title_full Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
title_fullStr Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
title_full_unstemmed Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
title_short Activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
title_sort activation and polarization of circulating monocytes in severe chronic obstructive pulmonary disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003040/
https://www.ncbi.nlm.nih.gov/pubmed/29907106
http://dx.doi.org/10.1186/s12890-018-0664-y
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