Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles

We assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emu...

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Detalles Bibliográficos
Autores principales: Tsugane, Mamiko, Suzuki, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003926/
https://www.ncbi.nlm.nih.gov/pubmed/29907779
http://dx.doi.org/10.1038/s41598-018-27547-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003926/
https://www.ncbi.nlm.nih.gov/pubmed/29907779
http://dx.doi.org/10.1038/s41598-018-27547-2

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