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Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles
We assessed the applicability of giant unilamellar vesicles (GUVs) for RNA detection using in vesicle reverse transcription polymerase chain reaction (RT-PCR). We prepared GUVs that encapsulated one-pot RT-PCR reaction mixture including template RNA, primers, and Taqman probe, using water-in-oil emu...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6003926/ https://www.ncbi.nlm.nih.gov/pubmed/29907779 http://dx.doi.org/10.1038/s41598-018-27547-2 |