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Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes

Cell-free systems are widely used to study mechanisms and regulation of translation, but the use of in vitro transcribed (IVT) mRNAs as translation substrates limits their efficiency and utility. Here, we present an approach for in vitro translation of messenger ribonucleoprotein (mRNP) complexes af...

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Detalles Bibliográficos
Autores principales: Fritz, Sarah E., Haque, Nazmul, Hogg, J. Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004058/
https://www.ncbi.nlm.nih.gov/pubmed/29724884
http://dx.doi.org/10.1261/rna.065730.118