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Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes
Cell-free systems are widely used to study mechanisms and regulation of translation, but the use of in vitro transcribed (IVT) mRNAs as translation substrates limits their efficiency and utility. Here, we present an approach for in vitro translation of messenger ribonucleoprotein (mRNP) complexes af...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004058/ https://www.ncbi.nlm.nih.gov/pubmed/29724884 http://dx.doi.org/10.1261/rna.065730.118 |