Cargando…

Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexes

Cell-free systems are widely used to study mechanisms and regulation of translation, but the use of in vitro transcribed (IVT) mRNAs as translation substrates limits their efficiency and utility. Here, we present an approach for in vitro translation of messenger ribonucleoprotein (mRNP) complexes af...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fritz, Sarah E., Haque, Nazmul, Hogg, J. Robert
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2018
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004058/ https://www.ncbi.nlm.nih.gov/pubmed/29724884 http://dx.doi.org/10.1261/rna.065730.118

Ejemplares similares

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
por: Kim, Sojung, et al.
Publicado: (2014)

Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactionsby Differential Affinity Immobilization
por: Jiang, Hua, et al.
Publicado: (2019)

A high-throughput pipeline for the production of synthetic antibodies for analysis of ribonucleoprotein complexes
por: Na, Hong, et al.
Publicado: (2016)

Ribonucleoprotein purification and characterization using RNA Mango
por: Panchapakesan, Shanker Shyam S., et al.
Publicado: (2017)

High-salt–recovered sequences are associated with the active chromosomal compartment and with large ribonucleoprotein complexes including nuclear bodies
por: Baudement, Marie-Odile, et al.
Publicado: (2018)

Neuronal messenger ribonucleoprotein transport follows an aging Lévy walk
por: Song, Minho S., et al.
Publicado: (2018)

Crystal structure of Tpa1 from Saccharomyces cerevisiae, a component of the messenger ribonucleoprotein complex
por: Kim, Hyoun Sook, et al.
Publicado: (2010)

A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules
por: Castillo, Kathrina D., et al.
Publicado: (2022)

An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes
por: Taoka, Masato, et al.
Publicado: (2009)

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
por: Liu, Qizhi, et al.
Publicado: (2018)

An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins
por: Leppek, Kathrin, et al.
Publicado: (2014)

Purification of Messenger Ribonucleoprotein Particles via a Tagged Nascent Polypeptide
por: Inchaustegui Gil, Diana P., et al.
Publicado: (2016)

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes
por: Zhou, Zhi, et al.
Publicado: (2017)

Mass spectrometry analysis of affinity-purified cytoplasmic translation initiation complexes from human and fly cells
por: Singh, Chingakham Ranjit, et al.
Publicado: (2022)

Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull
por: Rodgers, Margaret L., et al.
Publicado: (2015)

Messenger RNA processing and nuclear structure: isolation of nuclear ribonucleoprotein particles containing beta-globin messenger RNA precursors
Publicado: (1980)

Isolation and identification of cell-specific microRNAs targeting a messenger RNA using a biotinylated anti-sense oligonucleotide capture affinity technique
por: Hassan, Tidi, et al.
Publicado: (2013)

Protein kinase activity associated with stored messenger ribonucleoprotein particles of Xenopus oocytes
Publicado: (1988)

Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification
por: Imai, Hirotatsu, et al.
Publicado: (2023)

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing
por: Lyu, Pin, et al.
Publicado: (2019)

The Nucleolus and the Four Ribonucleoproteins of Translation
por: Pederson, Thoru, et al.
Publicado: (2000)

Computational Assessment of Protein–protein Binding Affinity by Reversely Engineering the Energetics in Protein Complexes
por: Wang, Bo, et al.
Publicado: (2021)

HOPS: automated detection and authentication of pathogen DNA in archaeological remains
por: Hübler, Ron, et al.
Publicado: (2019)

The Impact of Purifying and Background Selection on the Inference of Population History: Problems and Prospects
por: Johri, Parul, et al.
Publicado: (2021)

Large-scale Purification of Type III Toxin-antitoxin Ribonucleoprotein Complex and its Components from Escherichia coli for Biophysical Studies
por: Manikandan, Parthasarathy, et al.
Publicado: (2023)

Biogenesis, assembly, and export of viral messenger ribonucleoproteins in the influenza A virus infected cell
por: York, Ashley, et al.
Publicado: (2013)

Absence of the Fragile X messenger ribonucleoprotein alters response patterns to sounds in the auditory midbrain
por: Sibille, Jérémie, et al.
Publicado: (2022)

The effect of single-cell knockout of Fragile X Messenger Ribonucleoprotein on synaptic structural plasticity
por: Gredell, Marie, et al.
Publicado: (2023)

A highly efficient human cell-free translation system
por: Aleksashin, Nikolay A., et al.
Publicado: (2023)

A simple real-time assay for in vitro translation
por: Capece, Mark C., et al.
Publicado: (2015)

In vitro affinity maturation of antibody against membrane-bound GPCR molecules
por: Wang, Jie, et al.
Publicado: (2019)

Vanillin Inhibits Translation and Induces Messenger Ribonucleoprotein (mRNP) Granule Formation in Saccharomyces cerevisiae: Application and Validation of High-Content, Image-Based Profiling
por: Iwaki, Aya, et al.
Publicado: (2013)

Proteomic analysis of affinity-purified extracellular proteasomes reveals exclusively 20S complexes
por: Kulichkova, Valentina A., et al.
Publicado: (2017)

MethylPurify: tumor purity deconvolution and differential methylation detection from single tumor DNA methylomes
por: Zheng, Xiaoqi, et al.
Publicado: (2014)

Common ELIXIR Service for Researcher Authentication and Authorisation
por: Linden, Mikael, et al.
Publicado: (2018)

Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro
por: Trainor, Brandon M., et al.
Publicado: (2021)

Cytolethal distending toxin induces the formation of transient messenger-rich ribonucleoprotein nuclear invaginations in surviving cells
por: Azzi-Martin, Lamia, et al.
Publicado: (2019)

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein
por: Wu, Cheng, et al.
Publicado: (2023)

Affinity selection of DNA-binding protein complexes using mRNA display
por: Tateyama, Seiji, et al.
Publicado: (2006)

Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
por: Kühn-Hölsken, Eva, et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_mh1449i4ea9qvf9lt2mlp0ou1j