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Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study

A simple and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous determination of acetaminophen and oxycodone in human plasma. Acetaminophen-d4 and oxycodone-d3 were used as internal standards. The challenge encountered in the method de...

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Autores principales: Lu, Wei, Zhao, Shunbo, Gong, Meng, Sun, Luning, Ding, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004620/
https://www.ncbi.nlm.nih.gov/pubmed/29922484
http://dx.doi.org/10.1016/j.jpha.2018.01.006
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author Lu, Wei
Zhao, Shunbo
Gong, Meng
Sun, Luning
Ding, Li
author_facet Lu, Wei
Zhao, Shunbo
Gong, Meng
Sun, Luning
Ding, Li
author_sort Lu, Wei
collection PubMed
description A simple and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous determination of acetaminophen and oxycodone in human plasma. Acetaminophen-d4 and oxycodone-d3 were used as internal standards. The challenge encountered in the method development that the high plasma concentration level of acetaminophen made the MS response saturated while the desired lower limit of quantification (LLOQ) for oxycodone was hard to reach was well solved. The analytes were extracted by protein precipitation using acetonitrile. The matrix effect of the analytes was avoided by chromatographic separation using a hydrophilic C(18) column coupled with gradient elution. Multiple reaction monitoring in positive ion mode was performed on tandem mass spectrometer employing electrospray ion source. The calibration curves were linear over the concentration ranges of 40.0–8000 ng/mL and 0.200–40.0 ng/mL for acetaminophen and oxycodone, respectively. This method, which could contribute to high throughput analysis and better clinical drug monitoring, was successfully applied to a pharmacokinetic study in healthy Chinese volunteers.
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spelling pubmed-60046202018-06-19 Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study Lu, Wei Zhao, Shunbo Gong, Meng Sun, Luning Ding, Li J Pharm Anal Original Research Article A simple and rapid liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous determination of acetaminophen and oxycodone in human plasma. Acetaminophen-d4 and oxycodone-d3 were used as internal standards. The challenge encountered in the method development that the high plasma concentration level of acetaminophen made the MS response saturated while the desired lower limit of quantification (LLOQ) for oxycodone was hard to reach was well solved. The analytes were extracted by protein precipitation using acetonitrile. The matrix effect of the analytes was avoided by chromatographic separation using a hydrophilic C(18) column coupled with gradient elution. Multiple reaction monitoring in positive ion mode was performed on tandem mass spectrometer employing electrospray ion source. The calibration curves were linear over the concentration ranges of 40.0–8000 ng/mL and 0.200–40.0 ng/mL for acetaminophen and oxycodone, respectively. This method, which could contribute to high throughput analysis and better clinical drug monitoring, was successfully applied to a pharmacokinetic study in healthy Chinese volunteers. Xi'an Jiaotong University 2018-06 2018-01-31 /pmc/articles/PMC6004620/ /pubmed/29922484 http://dx.doi.org/10.1016/j.jpha.2018.01.006 Text en © 2018 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Lu, Wei
Zhao, Shunbo
Gong, Meng
Sun, Luning
Ding, Li
Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study
title Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study
title_full Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study
title_fullStr Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study
title_full_unstemmed Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study
title_short Simultaneous determination of acetaminophen and oxycodone in human plasma by LC–MS/MS and its application to a pharmacokinetic study
title_sort simultaneous determination of acetaminophen and oxycodone in human plasma by lc–ms/ms and its application to a pharmacokinetic study
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6004620/
https://www.ncbi.nlm.nih.gov/pubmed/29922484
http://dx.doi.org/10.1016/j.jpha.2018.01.006
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