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Experimental and bioinformatic approach to identifying antigenic epitopes in human α- and β-enolases
Human α- and β-enolases are highly homologous enzymes, difficult to differentiate immunologically. In this work, we describe production, purification and properties of anti-α- and anti-β-enolase polyclonal antibodies. To raise antibodies, rabbits were injected with enolase isoenzymes that were purif...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005794/ https://www.ncbi.nlm.nih.gov/pubmed/29922723 http://dx.doi.org/10.1016/j.bbrep.2018.05.008 |