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Effect of endogenous reference genes on digital PCR assessment of genetically engineered canola events
Droplet digital PCR (ddPCR) has been used for absolute quantification of genetically engineered (GE) events. Absolute quantification of GE events by duplex ddPCR requires the use of appropriate primers and probes for target and reference gene sequences in order to accurately determine the amount of...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006385/ https://www.ncbi.nlm.nih.gov/pubmed/29922591 http://dx.doi.org/10.1016/j.bdq.2018.03.002 |