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Effect of endogenous reference genes on digital PCR assessment of genetically engineered canola events

Droplet digital PCR (ddPCR) has been used for absolute quantification of genetically engineered (GE) events. Absolute quantification of GE events by duplex ddPCR requires the use of appropriate primers and probes for target and reference gene sequences in order to accurately determine the amount of...

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Detalles Bibliográficos
Autores principales: Demeke, Tigst, Eng, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006385/
https://www.ncbi.nlm.nih.gov/pubmed/29922591
http://dx.doi.org/10.1016/j.bdq.2018.03.002