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A PCR-based quantitative assay for the evaluation of mRNA integrity in rat samples
Reverse Transcription quantitative real-time PCR (RT-qPCR) is applied to quantify gene transcript levels in a wide range of investigations. Proper assessment of RNA integrity is essential for reliable assessment of gene expression levels, as RNA molecules are acutely vulnerable to degradation. Howev...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006387/ https://www.ncbi.nlm.nih.gov/pubmed/29922590 http://dx.doi.org/10.1016/j.bdq.2018.02.001 |