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A PCR-based quantitative assay for the evaluation of mRNA integrity in rat samples

Reverse Transcription quantitative real-time PCR (RT-qPCR) is applied to quantify gene transcript levels in a wide range of investigations. Proper assessment of RNA integrity is essential for reliable assessment of gene expression levels, as RNA molecules are acutely vulnerable to degradation. Howev...

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Detalles Bibliográficos
Autores principales: Padhi, Bhaja K., Singh, Manjeet, Rosales, Marianela, Pelletier, Guillaume, Cakmak, Sabit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006387/
https://www.ncbi.nlm.nih.gov/pubmed/29922590
http://dx.doi.org/10.1016/j.bdq.2018.02.001