Cargando…
A PCR-based quantitative assay for the evaluation of mRNA integrity in rat samples
Reverse Transcription quantitative real-time PCR (RT-qPCR) is applied to quantify gene transcript levels in a wide range of investigations. Proper assessment of RNA integrity is essential for reliable assessment of gene expression levels, as RNA molecules are acutely vulnerable to degradation. Howev...
Autores principales: | Padhi, Bhaja K., Singh, Manjeet, Rosales, Marianela, Pelletier, Guillaume, Cakmak, Sabit |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006387/ https://www.ncbi.nlm.nih.gov/pubmed/29922590 http://dx.doi.org/10.1016/j.bdq.2018.02.001 |
Ejemplares similares
-
Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells
por: Pelletier, Guillaume, et al.
Publicado: (2015) -
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR
por: Staines, Karen, et al.
Publicado: (2016) -
Evaluation of sense-strand mRNA amplification by comparative quantitative PCR
por: Goff, Loyal A, et al.
Publicado: (2004) -
Quantitative real-time PCR for the measurement of feline cytokine mRNA
por: Leutenegger, Christian M, et al.
Publicado: (1999) -
"Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays
por: Kanno, Jun, et al.
Publicado: (2006)