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Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies
The aim of this work was to develop a cryopreservation method of small liver biopsies for in situ mitochondrial function assessment. Herein we describe a detailed protocol for tissue collection, cryopreservation, high-resolution respirometry using complex I and II substrates, calculation and interpr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006522/ https://www.ncbi.nlm.nih.gov/pubmed/29704825 http://dx.doi.org/10.1016/j.redox.2018.03.008 |