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Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies

The aim of this work was to develop a cryopreservation method of small liver biopsies for in situ mitochondrial function assessment. Herein we describe a detailed protocol for tissue collection, cryopreservation, high-resolution respirometry using complex I and II substrates, calculation and interpr...

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Detalles Bibliográficos
Autores principales: García-Roche, Mercedes, Casal, Alberto, Carriquiry, Mariana, Radi, Rafael, Quijano, Celia, Cassina, Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006522/
https://www.ncbi.nlm.nih.gov/pubmed/29704825
http://dx.doi.org/10.1016/j.redox.2018.03.008