Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2,4-dinitrophenylhydrazine-based photometric assay

A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the...

Descripción completa

Detalles Bibliográficos
Autores principales: Georgiou, Christos D., Zisimopoulos, Dimitrios, Argyropoulou, Vasiliki, Kalaitzopoulou, Electra, Salachas, George, Grune, Tilman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006683/
https://www.ncbi.nlm.nih.gov/pubmed/29684819
http://dx.doi.org/10.1016/j.redox.2018.04.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006683/
https://www.ncbi.nlm.nih.gov/pubmed/29684819
http://dx.doi.org/10.1016/j.redox.2018.04.010

Ejemplares similares