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zUMIs - A fast and flexible pipeline to process RNA sequencing data with UMIs

BACKGROUND: Single-cell RNA-sequencing (scRNA-seq) experiments typically analyze hundreds or thousands of cells after amplification of the cDNA. The high throughput is made possible by the early introduction of sample-specific bar codes (BCs), and the amplification bias is alleviated by unique molec...

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Detalles Bibliográficos
Autores principales: Parekh, Swati, Ziegenhain, Christoph, Vieth, Beate, Enard, Wolfgang, Hellmann, Ines
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007394/
https://www.ncbi.nlm.nih.gov/pubmed/29846586
http://dx.doi.org/10.1093/gigascience/giy059