Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis

Haploid mouse embryonic stem cells (ESCs), in which a single hit mutation is sufficient to produce loss-of-function phenotypes, have provided a powerful tool for forward genetic screening. This strategy, however, can be hampered by undesired autodiploidization of haploid ESCs. To overcome this obsta...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamanishi, Ayako, Matsuba, Atsushi, Kondo, Ryohei, Akamatsu, Rie, Tanaka, Sachiyo, Tokunaga, Masahiro, Horie, Kyoji, Kokubu, Chikara, Ishida, Yasumasa, Takeda, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007410/
https://www.ncbi.nlm.nih.gov/pubmed/29554276
http://dx.doi.org/10.1093/nar/gky183

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6007410/
https://www.ncbi.nlm.nih.gov/pubmed/29554276
http://dx.doi.org/10.1093/nar/gky183

Ejemplares similares