Rapid Purification of Nuclei from Animal and Plant Tissues and Cultured Cells

Nuclei are isolated by buoyant density banding in a discontinuous iodixanol gradient, under isoosmotic conditions. The low viscosity of the gradient allows the purification to be carried out at 10,000g in only 20 min. The method avoids possible damage to nucleoprotein complexes caused by hyperosmoti...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009280/
https://www.ncbi.nlm.nih.gov/pubmed/12806139
http://dx.doi.org/10.1100/tsw.2002.832

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009280/
https://www.ncbi.nlm.nih.gov/pubmed/12806139
http://dx.doi.org/10.1100/tsw.2002.832

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