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Rapid Purification of Nuclei from Animal and Plant Tissues and Cultured Cells
Nuclei are isolated by buoyant density banding in a discontinuous iodixanol gradient, under isoosmotic conditions. The low viscosity of the gradient allows the purification to be carried out at 10,000g in only 20 min. The method avoids possible damage to nucleoprotein complexes caused by hyperosmoti...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009280/ https://www.ncbi.nlm.nih.gov/pubmed/12806139 http://dx.doi.org/10.1100/tsw.2002.832 |