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Purification of Lipid Rafts from Cultured Cells
Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutio...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009391/ https://www.ncbi.nlm.nih.gov/pubmed/12806159 http://dx.doi.org/10.1100/tsw.2002.846 |