Purification of Lipid Rafts from Cultured Cells

Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutio...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009391/
https://www.ncbi.nlm.nih.gov/pubmed/12806159
http://dx.doi.org/10.1100/tsw.2002.846
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author Graham, John
author_facet Graham, John
author_sort Graham, John
collection PubMed
description Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutions contain 0.5–1.0% Triton X-100, the intact lipid rafts float to the top of the gradient while all of the other detergent-solubilized membranes remain at the bottom.
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spelling pubmed-60093912018-07-04 Purification of Lipid Rafts from Cultured Cells Graham, John ScientificWorldJournal Peer-Reviewed Protocol Lipid-rich lipid rafts are microdomains of the plasma membrane that are resistant to low concentrations of nonionic detergent. This forms the basis for their isolation. Either a microsomal fraction or a postnuclear supernatant are loaded beneath a discontinuous iodixanol gradient. If all the solutions contain 0.5–1.0% Triton X-100, the intact lipid rafts float to the top of the gradient while all of the other detergent-solubilized membranes remain at the bottom. TheScientificWorldJOURNAL 2002-06-18 /pmc/articles/PMC6009391/ /pubmed/12806159 http://dx.doi.org/10.1100/tsw.2002.846 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Purification of Lipid Rafts from Cultured Cells
title Purification of Lipid Rafts from Cultured Cells
title_full Purification of Lipid Rafts from Cultured Cells
title_fullStr Purification of Lipid Rafts from Cultured Cells
title_full_unstemmed Purification of Lipid Rafts from Cultured Cells
title_short Purification of Lipid Rafts from Cultured Cells
title_sort purification of lipid rafts from cultured cells
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009391/
https://www.ncbi.nlm.nih.gov/pubmed/12806159
http://dx.doi.org/10.1100/tsw.2002.846
work_keys_str_mv AT grahamjohn purificationoflipidraftsfromculturedcells

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