Attachment of Histidine Tags to Recombinant Tumor Necrosis Factor-Alpha Drastically Changes Its Properties

When studying two different histidine tags attached to the N-termini of the trimeric cytokine tumor necrosis factor alpha (TNF), the biological activity — measured as cytotoxicity on the L-929 cell line — of both tagged proteins was drastically reduced. The longer His10 tag reduced cytotoxicity to a...

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Detalles Bibliográficos
Autores principales: Fonda, Irena, Kenig, Maja, Gaberc-Porekar, Vladka, Pristovaek, Primo, Menart, Viktor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009408/
https://www.ncbi.nlm.nih.gov/pubmed/12805914
http://dx.doi.org/10.1100/tsw.2002.215

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009408/
https://www.ncbi.nlm.nih.gov/pubmed/12805914
http://dx.doi.org/10.1100/tsw.2002.215

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