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Attachment of Histidine Tags to Recombinant Tumor Necrosis Factor-Alpha Drastically Changes Its Properties
When studying two different histidine tags attached to the N-termini of the trimeric cytokine tumor necrosis factor alpha (TNF), the biological activity — measured as cytotoxicity on the L-929 cell line — of both tagged proteins was drastically reduced. The longer His10 tag reduced cytotoxicity to a...
Autores principales: | Fonda, Irena, Kenig, Maja, Gaberc-Porekar, Vladka, Pristovaek, Primo, Menart, Viktor |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
TheScientificWorldJOURNAL
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009408/ https://www.ncbi.nlm.nih.gov/pubmed/12805914 http://dx.doi.org/10.1100/tsw.2002.215 |
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