Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor

In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose an...

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Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009509/
https://www.ncbi.nlm.nih.gov/pubmed/12806135
http://dx.doi.org/10.1100/tsw.2002.838
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author Graham, John
author_facet Graham, John
author_sort Graham, John
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description In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll® there is no contamination from endoplasmic reticulum.
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spelling pubmed-60095092018-07-04 Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor Graham, John ScientificWorldJournal Peer-Reviewed Protocol In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll® there is no contamination from endoplasmic reticulum. TheScientificWorldJOURNAL 2002-06-07 /pmc/articles/PMC6009509/ /pubmed/12806135 http://dx.doi.org/10.1100/tsw.2002.838 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
title Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
title_full Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
title_fullStr Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
title_full_unstemmed Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
title_short Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
title_sort purification of peroxisomes in a preformed iodixanol gradient in a fixed-angle rotor
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009509/
https://www.ncbi.nlm.nih.gov/pubmed/12806135
http://dx.doi.org/10.1100/tsw.2002.838
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