Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose an...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009509/ https://www.ncbi.nlm.nih.gov/pubmed/12806135 http://dx.doi.org/10.1100/tsw.2002.838 |
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author | Graham, John |
author_facet | Graham, John |
author_sort | Graham, John |
collection | PubMed |
description | In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll® there is no contamination from endoplasmic reticulum. |
format | Online Article Text |
id | pubmed-6009509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | TheScientificWorldJOURNAL |
record_format | MEDLINE/PubMed |
spelling | pubmed-60095092018-07-04 Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor Graham, John ScientificWorldJournal Peer-Reviewed Protocol In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a preformed isosmotic continuous gradient. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in Percoll® there is no contamination from endoplasmic reticulum. TheScientificWorldJOURNAL 2002-06-07 /pmc/articles/PMC6009509/ /pubmed/12806135 http://dx.doi.org/10.1100/tsw.2002.838 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Peer-Reviewed Protocol Graham, John Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor |
title | Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor |
title_full | Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor |
title_fullStr | Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor |
title_full_unstemmed | Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor |
title_short | Purification of Peroxisomes in a Preformed Iodixanol Gradient in a Fixed-Angle Rotor |
title_sort | purification of peroxisomes in a preformed iodixanol gradient in a fixed-angle rotor |
topic | Peer-Reviewed Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009509/ https://www.ncbi.nlm.nih.gov/pubmed/12806135 http://dx.doi.org/10.1100/tsw.2002.838 |
work_keys_str_mv | AT grahamjohn purificationofperoxisomesinapreformediodixanolgradientinafixedanglerotor |