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Viral attenuation by engineered protein fragmentation

A possible but untested method of viral attenuation is protein fragmentation, engineering wild-type proteins as two or more peptides that self-assemble after translation. Here, the bacteriophage T7 was engineered to encode its essential RNA polymerase as two peptides. Initial fitness was profoundly...

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Detalles Bibliográficos
Autores principales: Garry, Daniel J, Ellington, Andrew D, Molineux, Ian J, Bull, James J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009699/
https://www.ncbi.nlm.nih.gov/pubmed/29942657
http://dx.doi.org/10.1093/ve/vey017