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Isolation of Human Polymorphonuclear Leukocytes (Granulocytes) from a Leukocyte-Rich Fraction
Human peripheral blood polymorphonuclear leukocytes (PMNs) or granulocytes from a leukocyte-rich plasma (LRP) are banded at an interface between two layers of iodixanol. If the denser layer of iodixanol is omitted the PMNs may alternatively be pelleted. The procedure can be adapted to blood from oth...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009719/ https://www.ncbi.nlm.nih.gov/pubmed/12805923 http://dx.doi.org/10.1100/tsw.2002.831 |