Separation of Membrane Vesicles and Cytosol from Yeast, Cultured Cells, and Bacteria in a Small Volume Self-Generated Gradient in a Fixed-Angle Rotor

There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from yeast, cultured cells, or from bacteria. This Protocol Article describes the flotation of the vesicles through a self-generated gradient from a dense sample zone using...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009728/
https://www.ncbi.nlm.nih.gov/pubmed/12806146
http://dx.doi.org/10.1100/tsw.2002.833

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009728/
https://www.ncbi.nlm.nih.gov/pubmed/12806146
http://dx.doi.org/10.1100/tsw.2002.833

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