Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient

There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009740/
https://www.ncbi.nlm.nih.gov/pubmed/12806140
http://dx.doi.org/10.1100/tsw.2002.834

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009740/
https://www.ncbi.nlm.nih.gov/pubmed/12806140
http://dx.doi.org/10.1100/tsw.2002.834

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