Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient

There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows...

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Detalles Bibliográficos
Autor principal: Graham, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2002
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009740/
https://www.ncbi.nlm.nih.gov/pubmed/12806140
http://dx.doi.org/10.1100/tsw.2002.834
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author Graham, John
author_facet Graham, John
author_sort Graham, John
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description There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows complete separation of these compartments. As the sample is exposed to the gmax the tendency of the proteins to sediment overcomes any diffusion in the opposite direction.
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spelling pubmed-60097402018-07-04 Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient Graham, John ScientificWorldJournal Peer-Reviewed Protocol There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows complete separation of these compartments. As the sample is exposed to the gmax the tendency of the proteins to sediment overcomes any diffusion in the opposite direction. TheScientificWorldJOURNAL 2002-06-07 /pmc/articles/PMC6009740/ /pubmed/12806140 http://dx.doi.org/10.1100/tsw.2002.834 Text en Copyright © 2002 John Graham. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Graham, John
Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
title Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
title_full Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
title_fullStr Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
title_full_unstemmed Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
title_short Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
title_sort separation of membrane vesicles and cytosol from cultured cells and bacteria in a preformed discontinuous gradient
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009740/
https://www.ncbi.nlm.nih.gov/pubmed/12806140
http://dx.doi.org/10.1100/tsw.2002.834
work_keys_str_mv AT grahamjohn separationofmembranevesiclesandcytosolfromculturedcellsandbacteriainapreformeddiscontinuousgradient

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