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Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient
There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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TheScientificWorldJOURNAL
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6009740/ https://www.ncbi.nlm.nih.gov/pubmed/12806140 http://dx.doi.org/10.1100/tsw.2002.834 |