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CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages

CD38 was first identified as a lymphocyte-specific antigen and then has been found to be widely expressed in a variety of cell types. The functions of CD38 are involved in numerous biological processes including immune responses. Here, we showed the downregulations of both TLR2 mRNA and protein in m...

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Autores principales: Qian, Yisong, Chen, Chuqiao, Ma, Leliang, Wang, Ziwei, Wang, Ling-Fang, Zuo, Li, Yang, Yaqin, Huang, Xiang, Jiang, Meixiu, Wang, Xiaolei, Shi, Huidong, Fu, Mingui, Deng, Ke-Yu, Xin, Hong-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011090/
https://www.ncbi.nlm.nih.gov/pubmed/29977153
http://dx.doi.org/10.1155/2018/8736949
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author Qian, Yisong
Chen, Chuqiao
Ma, Leliang
Wang, Ziwei
Wang, Ling-Fang
Zuo, Li
Yang, Yaqin
Huang, Xiang
Jiang, Meixiu
Wang, Xiaolei
Shi, Huidong
Fu, Mingui
Deng, Ke-Yu
Xin, Hong-Bo
author_facet Qian, Yisong
Chen, Chuqiao
Ma, Leliang
Wang, Ziwei
Wang, Ling-Fang
Zuo, Li
Yang, Yaqin
Huang, Xiang
Jiang, Meixiu
Wang, Xiaolei
Shi, Huidong
Fu, Mingui
Deng, Ke-Yu
Xin, Hong-Bo
author_sort Qian, Yisong
collection PubMed
description CD38 was first identified as a lymphocyte-specific antigen and then has been found to be widely expressed in a variety of cell types. The functions of CD38 are involved in numerous biological processes including immune responses. Here, we showed the downregulations of both TLR2 mRNA and protein in macrophages from CD38(−/−) mice and in CD38 knockdown RAW264.7 cells. Several NF-κB-binding motifs in the promoter region of the TLR2 gene were identified by the bioinformatics analysis and were confirmed by the luciferase activity assay with the different truncated TLR2 promoters. CD38 deficiency resulted in the reduction of NF-κB p65 and acetyl-NF-κB p65 (Ac-p65) levels as determined by Western blot. The expression of Sirt1 did not change, but an increased activity of Sirt1 was observed in CD38-deficient macrophages. Inhibition of the Sirt1/NF-κB signaling pathway resulted in downregulation of TLR2 expression in RAW264.7 cells. However, re-expression of CD38 in the knockdown clones reversed the effect on Sirt1/NF-κB/TLR2 signaling, which is NAD-dependent. Moreover, the inflammatory cytokines including G-CSF, IL-1alpha, IL-6, MCP-1, MIP-1alpha, and RANTES were increased in CD38 knockdown RAW264.7 cells. Taken together, our data demonstrated that CD38 deficiency enhances inflammatory response in macrophages, and the mechanism may be partly associated with increased Sirt1 activity, which promoted NF-κB deacetylation and then inhibited expression of the TLR2 gene. Obviously, our study may provide an insight into the molecular mechanisms in CD38-mediated inflammation.
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spelling pubmed-60110902018-07-05 CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages Qian, Yisong Chen, Chuqiao Ma, Leliang Wang, Ziwei Wang, Ling-Fang Zuo, Li Yang, Yaqin Huang, Xiang Jiang, Meixiu Wang, Xiaolei Shi, Huidong Fu, Mingui Deng, Ke-Yu Xin, Hong-Bo Mediators Inflamm Research Article CD38 was first identified as a lymphocyte-specific antigen and then has been found to be widely expressed in a variety of cell types. The functions of CD38 are involved in numerous biological processes including immune responses. Here, we showed the downregulations of both TLR2 mRNA and protein in macrophages from CD38(−/−) mice and in CD38 knockdown RAW264.7 cells. Several NF-κB-binding motifs in the promoter region of the TLR2 gene were identified by the bioinformatics analysis and were confirmed by the luciferase activity assay with the different truncated TLR2 promoters. CD38 deficiency resulted in the reduction of NF-κB p65 and acetyl-NF-κB p65 (Ac-p65) levels as determined by Western blot. The expression of Sirt1 did not change, but an increased activity of Sirt1 was observed in CD38-deficient macrophages. Inhibition of the Sirt1/NF-κB signaling pathway resulted in downregulation of TLR2 expression in RAW264.7 cells. However, re-expression of CD38 in the knockdown clones reversed the effect on Sirt1/NF-κB/TLR2 signaling, which is NAD-dependent. Moreover, the inflammatory cytokines including G-CSF, IL-1alpha, IL-6, MCP-1, MIP-1alpha, and RANTES were increased in CD38 knockdown RAW264.7 cells. Taken together, our data demonstrated that CD38 deficiency enhances inflammatory response in macrophages, and the mechanism may be partly associated with increased Sirt1 activity, which promoted NF-κB deacetylation and then inhibited expression of the TLR2 gene. Obviously, our study may provide an insight into the molecular mechanisms in CD38-mediated inflammation. Hindawi 2018-05-27 /pmc/articles/PMC6011090/ /pubmed/29977153 http://dx.doi.org/10.1155/2018/8736949 Text en Copyright © 2018 Yisong Qian et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qian, Yisong
Chen, Chuqiao
Ma, Leliang
Wang, Ziwei
Wang, Ling-Fang
Zuo, Li
Yang, Yaqin
Huang, Xiang
Jiang, Meixiu
Wang, Xiaolei
Shi, Huidong
Fu, Mingui
Deng, Ke-Yu
Xin, Hong-Bo
CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages
title CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages
title_full CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages
title_fullStr CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages
title_full_unstemmed CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages
title_short CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages
title_sort cd38 deficiency promotes inflammatory response through activating sirt1/nf-κb-mediated inhibition of tlr2 expression in macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011090/
https://www.ncbi.nlm.nih.gov/pubmed/29977153
http://dx.doi.org/10.1155/2018/8736949
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