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BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity

The mesodermal germ layer is patterned into mediolateral subtypes by signaling factors including BMP and FGF. How these pathways are integrated to induce specific mediolateral cell fates is not well understood. We used mesoderm derived from post-gastrulation neuromesodermal progenitors (NMPs), which...

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Autores principales: Row, Richard H, Pegg, Amy, Kinney, Brian A, Farr, Gist H, Maves, Lisa, Lowell, Sally, Wilson, Valerie, Martin, Benjamin L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013256/
https://www.ncbi.nlm.nih.gov/pubmed/29877796
http://dx.doi.org/10.7554/eLife.31018
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author Row, Richard H
Pegg, Amy
Kinney, Brian A
Farr, Gist H
Maves, Lisa
Lowell, Sally
Wilson, Valerie
Martin, Benjamin L
author_facet Row, Richard H
Pegg, Amy
Kinney, Brian A
Farr, Gist H
Maves, Lisa
Lowell, Sally
Wilson, Valerie
Martin, Benjamin L
author_sort Row, Richard H
collection PubMed
description The mesodermal germ layer is patterned into mediolateral subtypes by signaling factors including BMP and FGF. How these pathways are integrated to induce specific mediolateral cell fates is not well understood. We used mesoderm derived from post-gastrulation neuromesodermal progenitors (NMPs), which undergo a binary mediolateral patterning decision, as a simplified model to understand how FGF acts together with BMP to impart mediolateral fate. Using zebrafish and mouse NMPs, we identify an evolutionarily conserved mechanism of BMP and FGF-mediated mediolateral mesodermal patterning that occurs through modulation of basic helix-loop-helix (bHLH) transcription factor activity. BMP imparts lateral fate through induction of Id helix loop helix (HLH) proteins, which antagonize bHLH transcription factors, induced by FGF signaling, that specify medial fate. We extend our analysis of zebrafish development to show that bHLH activity is responsible for the mediolateral patterning of the entire mesodermal germ layer.
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spelling pubmed-60132562018-06-25 BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity Row, Richard H Pegg, Amy Kinney, Brian A Farr, Gist H Maves, Lisa Lowell, Sally Wilson, Valerie Martin, Benjamin L eLife Developmental Biology The mesodermal germ layer is patterned into mediolateral subtypes by signaling factors including BMP and FGF. How these pathways are integrated to induce specific mediolateral cell fates is not well understood. We used mesoderm derived from post-gastrulation neuromesodermal progenitors (NMPs), which undergo a binary mediolateral patterning decision, as a simplified model to understand how FGF acts together with BMP to impart mediolateral fate. Using zebrafish and mouse NMPs, we identify an evolutionarily conserved mechanism of BMP and FGF-mediated mediolateral mesodermal patterning that occurs through modulation of basic helix-loop-helix (bHLH) transcription factor activity. BMP imparts lateral fate through induction of Id helix loop helix (HLH) proteins, which antagonize bHLH transcription factors, induced by FGF signaling, that specify medial fate. We extend our analysis of zebrafish development to show that bHLH activity is responsible for the mediolateral patterning of the entire mesodermal germ layer. eLife Sciences Publications, Ltd 2018-06-07 /pmc/articles/PMC6013256/ /pubmed/29877796 http://dx.doi.org/10.7554/eLife.31018 Text en © 2018, Row et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Row, Richard H
Pegg, Amy
Kinney, Brian A
Farr, Gist H
Maves, Lisa
Lowell, Sally
Wilson, Valerie
Martin, Benjamin L
BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
title BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
title_full BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
title_fullStr BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
title_full_unstemmed BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
title_short BMP and FGF signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
title_sort bmp and fgf signaling interact to pattern mesoderm by controlling basic helix-loop-helix transcription factor activity
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013256/
https://www.ncbi.nlm.nih.gov/pubmed/29877796
http://dx.doi.org/10.7554/eLife.31018
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