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A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation
Reconstitution of membrane proteins in artificial membranes is an essential prerequisite for functional studies that depend on the context of an intact membrane. While straight-forward protocols for reconstituting proteins in small unilamellar vesicles were developed many years ago, it is much more...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013450/ https://www.ncbi.nlm.nih.gov/pubmed/29930377 http://dx.doi.org/10.1038/s41598-018-27456-4 |