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A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation

Reconstitution of membrane proteins in artificial membranes is an essential prerequisite for functional studies that depend on the context of an intact membrane. While straight-forward protocols for reconstituting proteins in small unilamellar vesicles were developed many years ago, it is much more...

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Detalles Bibliográficos
Autores principales: Witkowska, Agata, Jablonski, Lukasz, Jahn, Reinhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013450/
https://www.ncbi.nlm.nih.gov/pubmed/29930377
http://dx.doi.org/10.1038/s41598-018-27456-4