Cargando…

Bacterial Production, Characterization and Protein Modeling of a Novel Monofuctional Isoform of FAD Synthase in Humans: An Emergency Protein?

FAD synthase (FADS, EC 2.7.7.2) is the last essential enzyme involved in the pathway of biosynthesis of Flavin cofactors starting from Riboflavin (Rf). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. Besides the well characterized isoform 1 and 2...

Descripción completa

Detalles Bibliográficos
Autores principales:	Leone, Piero, Galluccio, Michele, Barbiroli, Alberto, Eberini, Ivano, Tolomeo, Maria, Vrenna, Flavia, Gianazza, Elisabetta, Iametti, Stefania, Bonomi, Francesco, Indiveri, Cesare, Barile, Maria
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017331/ https://www.ncbi.nlm.nih.gov/pubmed/29316637 http://dx.doi.org/10.3390/molecules23010116

Ejemplares similares

Mutation of Aspartate 238 in FAD Synthase Isoform 6 Increases the Specific Activity by Weakening the FAD Binding
por: Leone, Piero, et al.
Publicado: (2019)

Bacterial Over-Expression and Purification of the 3′phosphoadenosine 5′phosphosulfate (PAPS) Reductase Domain of Human FAD Synthase: Functional Characterization and Homology Modeling
por: Miccolis, Angelica, et al.
Publicado: (2012)

In silico Description of LAT1 Transport Mechanism at an Atomistic Level
por: Palazzolo, Luca, et al.
Publicado: (2018)

Beta-Lactoglobulin as a Model Food Protein: How to Promote, Prevent, and Exploit Its Unfolding Processes
por: Barbiroli, Alberto, et al.
Publicado: (2022)

Protein interactions in the biological assembly of iron–sulfur clusters in Escherichia coli : Molecular and mechanistic aspects of the earliest assembly steps
por: Bonomi, Francesco, et al.
Publicado: (2022)

Molecular Mechanism of Inhibition of the Mitochondrial Carnitine/Acylcarnitine Transporter by Omeprazole Revealed by Proteoliposome Assay, Mutagenesis and Bioinformatics
por: Tonazzi, Annamaria, et al.
Publicado: (2013)

Subcellular Localization of Fad1p in Saccharomyces cerevisiae: A Choice at Post-Transcriptional Level?
por: Bruni, Francesco, et al.
Publicado: (2021)

Mimicking human riboflavin responsive neuromuscular disorders by silencing flad‐1 gene in C. elegans : Alteration of vitamin transport and cholinergic transmission
por: Leone, Piero, et al.
Publicado: (2021)

Reconstitution in Proteoliposomes of the Recombinant Human Riboflavin Transporter 2 (SLC52A2) Overexpressed in E. coli
por: Console, Lara, et al.
Publicado: (2019)

Inhibition of Pancreatic α-amylase by Resveratrol Derivatives: Biological Activity and Molecular Modelling Evidence for Cooperativity between Viniferin Enantiomers
por: Mattio, Luce M., et al.
Publicado: (2019)

All-Purpose Containers? Lipid-Binding Protein – Drug Interactions
por: Beringhelli, Tiziana, et al.
Publicado: (2015)

Development of Novel Experimental Models to Study Flavoproteome Alterations in Human Neuromuscular Diseases: The Effect of Rf Therapy
por: Tolomeo, Maria, et al.
Publicado: (2020)

Pasta from Red Lentils (Lens culinaris): The Effect of Pasta-Making Process on Starch and Protein Features, and Cooking Behavior
por: Bresciani, Andrea, et al.
Publicado: (2022)

rHDL modeling and the anchoring mechanism of LCAT activation
por: Laurenzi, Tommaso, et al.
Publicado: (2020)

Remaining challenges in cellular flavin cofactor homeostasis and flavoprotein biogenesis
por: Giancaspero, Teresa A., et al.
Publicado: (2015)

Impact of Thermal Treatment on the Starch-Protein Interplay in Red Lentils: Connecting Molecular Features and Rheological Properties
por: Bresciani, Andrea, et al.
Publicado: (2022)

Cofactors and pathogens: Flavin mononucleotide and flavin adenine dinucleotide (FAD) biosynthesis by the FAD synthase from Brucella ovis
por: Moreno, Andrea, et al.
Publicado: (2021)

Riboflavin-Responsive and -Non-responsive Mutations in FAD Synthase Cause Multiple Acyl-CoA Dehydrogenase and Combined Respiratory-Chain Deficiency
por: Olsen, Rikke K.J., et al.
Publicado: (2016)

Sakacin‐A antimicrobial packaging for decreasing Listeria contamination in thin‐cut meat: preliminary assessment
por: Barbiroli, Alberto, et al.
Publicado: (2016)

Strategies of Bacterial Over Expression of Membrane Transporters Relevant in Human Health: The Successful Case of the Three Members of OCTN Subfamily
por: Indiveri, Cesare, et al.
Publicado: (2012)

Membrane transporters for the special amino acid glutamine: structure/function relationships and relevance to human health
por: Pochini, Lorena, et al.
Publicado: (2014)

Evolutionary divergence of chloroplast FAD synthetase proteins
por: Yruela, Inmaculada, et al.
Publicado: (2010)

Glutamine Transport and Mitochondrial Metabolism in Cancer Cell Growth
por: Scalise, Mariafrancesca, et al.
Publicado: (2017)

The Human SLC7A5 (LAT1): The Intriguing Histidine/Large Neutral Amino Acid Transporter and Its Relevance to Human Health
por: Scalise, Mariafrancesca, et al.
Publicado: (2018)

Low temperature bacterial expression of the neutral amino acid transporters SLC1A5 (ASCT2), and SLC6A19 (B0AT1)
por: Galluccio, Michele, et al.
Publicado: (2020)

Chemical Targeting of Membrane Transporters: Insights into Structure/Function Relationships
por: Scalise, Mariafrancesca, et al.
Publicado: (2020)

Glutamine transporters as pharmacological targets: From function to drug design
por: Scalise, Mariafrancesca, et al.
Publicado: (2020)

Development of the first in vivo GPR17 ligand through an iterative drug discovery pipeline: A novel disease-modifying strategy for multiple sclerosis
por: Parravicini, Chiara, et al.
Publicado: (2020)

Characterisation of protein isoforms encoded by the Drosophila Glycogen Synthase Kinase 3 gene shaggy
por: Korona, Dagmara, et al.
Publicado: (2020)

The Nutraceutical Alliin From Garlic Is a Novel Substrate of the Essential Amino Acid Transporter LAT1 (SLC7A5)
por: Scanga, Raffaella, et al.
Publicado: (2022)

Insights into the FMNAT Active Site of FAD Synthase: Aromaticity Is Essential for Flavin Binding and Catalysis
por: Serrano, Ana, et al.
Publicado: (2020)

The Link Between the Mitochondrial Fatty Acid Oxidation Derangement and Kidney Injury
por: Console, Lara, et al.
Publicado: (2020)

Dietary Polyphenols and In Vitro Intestinal Fructose Uptake and Transport: A Systematic Literature Review
por: Iametti, Stefania, et al.
Publicado: (2022)

Regulatory Aspects of the Vacuolar CAT2 Arginine Transporter of S. lycopersicum: Role of Osmotic Pressure and Cations
por: Cosco, Jessica, et al.
Publicado: (2019)

Membrane Transporters for Amino Acids as Players of Cancer Metabolic Rewiring
por: Scalise, Mariafrancesca, et al.
Publicado: (2020)

Effect of Cholesterol on the Organic Cation Transporter OCTN1 (SLC22A4)
por: Pochini, Lorena, et al.
Publicado: (2020)

Chemical Approaches for Studying the Biology and Pharmacology of Membrane Transporters: The Histidine/Large Amino Acid Transporter SLC7A5 as a Benchmark
por: Scalise, Mariafrancesca, et al.
Publicado: (2021)

OCTN1: A Widely Studied but Still Enigmatic Organic Cation Transporter Linked to Human Pathology and Drug Interactions
por: Pochini, Lorena, et al.
Publicado: (2022)

Strategies for Successful Over-Expression of Human Membrane Transport Systems Using Bacterial Hosts: Future Perspectives
por: Galluccio, Michele, et al.
Publicado: (2022)

FALLING FOR FADS? Diagnostic and therapeutic fads in Psychiatry
por: Jesus, S., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_vr5m15ihokvmfnc48eeun3rs36