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Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification
CRISPR/Cas9 is a highly efficient genome engineering tool, yet its off-target effects and sequence-dependent cleavage activity across different sgRNAs remain major concerns for its application. Here, we propose a nicking triggered exponential amplification reaction (NTEXPAR), a fast and sensitive in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018437/ https://www.ncbi.nlm.nih.gov/pubmed/30155144 http://dx.doi.org/10.1039/c6sc01355d |