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Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification

CRISPR/Cas9 is a highly efficient genome engineering tool, yet its off-target effects and sequence-dependent cleavage activity across different sgRNAs remain major concerns for its application. Here, we propose a nicking triggered exponential amplification reaction (NTEXPAR), a fast and sensitive in...

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Detalles Bibliográficos
Autores principales:	Zhang, Kaixiang, Deng, Ruijie, Li, Yue, Zhang, Ling, Li, Jinghong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2016
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018437/ https://www.ncbi.nlm.nih.gov/pubmed/30155144 http://dx.doi.org/10.1039/c6sc01355d

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