Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

To fully exploit the microbial genome resources, a high-throughput experimental platform is needed to associate genes with phenotypes at the genome level. We present here a novel method that enables investigation of the cellular consequences of repressing individual transcripts based on the CRISPR i...

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Detalles Bibliográficos
Autores principales: Wang, Tianmin, Guan, Changge, Guo, Jiahui, Liu, Bing, Wu, Yinan, Xie, Zhen, Zhang, Chong, Xing, Xin-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018678/
https://www.ncbi.nlm.nih.gov/pubmed/29946130
http://dx.doi.org/10.1038/s41467-018-04899-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018678/
https://www.ncbi.nlm.nih.gov/pubmed/29946130
http://dx.doi.org/10.1038/s41467-018-04899-x

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