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Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice

Gelatin is an anti‐inflammatory dietary component, and its predominant metabolites entering circulation are prolyl‐hydroxyproline (Pro‐Hyp) and glycine. We evaluated the protective effects of orally administered gelatin, glycine, and Pro‐Hyp 10:3:0.8 (w/w/w) against dextran sodium sulfate (DSS)‐indu...

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Autores principales: Zhu, Suqin, Huang, Min, Feng, Guangxin, Miao, Yu, Wu, Haohao, Zeng, Mingyong, Lo, Yangming Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021736/
https://www.ncbi.nlm.nih.gov/pubmed/29983966
http://dx.doi.org/10.1002/fsn3.639
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author Zhu, Suqin
Huang, Min
Feng, Guangxin
Miao, Yu
Wu, Haohao
Zeng, Mingyong
Lo, Yangming Martin
author_facet Zhu, Suqin
Huang, Min
Feng, Guangxin
Miao, Yu
Wu, Haohao
Zeng, Mingyong
Lo, Yangming Martin
author_sort Zhu, Suqin
collection PubMed
description Gelatin is an anti‐inflammatory dietary component, and its predominant metabolites entering circulation are prolyl‐hydroxyproline (Pro‐Hyp) and glycine. We evaluated the protective effects of orally administered gelatin, glycine, and Pro‐Hyp 10:3:0.8 (w/w/w) against dextran sodium sulfate (DSS)‐induced colitis in mice. According to clinical, histological, and biochemical parameters, they exhibited significant activities in the order of gelatin < glycine < Pro‐Hyp. Gelatin prevented the DSS‐induced increase in interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) in the colon, rather than in peripheral blood. Glycine and Pro‐Hyp attenuated the DSS‐induced rise in colonic IL‐6 and TNF‐α, as well as peripheral IL‐1β, IL‐6, and TNF‐α. Hematologic results show the attenuation of DSS‐induced leukocytosis and lymphocytosis by glycine and Pro‐Hyp, rather than gelatin. These findings suggest that glycine and Pro‐Hyp constitute the material basis for gelatin's anticolitis efficacy, and they have better anticolitis activities and distinct mechanisms of action when ingested as free compounds than as part of gelatin.
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spelling pubmed-60217362018-07-06 Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice Zhu, Suqin Huang, Min Feng, Guangxin Miao, Yu Wu, Haohao Zeng, Mingyong Lo, Yangming Martin Food Sci Nutr Original Research Gelatin is an anti‐inflammatory dietary component, and its predominant metabolites entering circulation are prolyl‐hydroxyproline (Pro‐Hyp) and glycine. We evaluated the protective effects of orally administered gelatin, glycine, and Pro‐Hyp 10:3:0.8 (w/w/w) against dextran sodium sulfate (DSS)‐induced colitis in mice. According to clinical, histological, and biochemical parameters, they exhibited significant activities in the order of gelatin < glycine < Pro‐Hyp. Gelatin prevented the DSS‐induced increase in interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6), and tumor necrosis factor‐α (TNF‐α) in the colon, rather than in peripheral blood. Glycine and Pro‐Hyp attenuated the DSS‐induced rise in colonic IL‐6 and TNF‐α, as well as peripheral IL‐1β, IL‐6, and TNF‐α. Hematologic results show the attenuation of DSS‐induced leukocytosis and lymphocytosis by glycine and Pro‐Hyp, rather than gelatin. These findings suggest that glycine and Pro‐Hyp constitute the material basis for gelatin's anticolitis efficacy, and they have better anticolitis activities and distinct mechanisms of action when ingested as free compounds than as part of gelatin. John Wiley and Sons Inc. 2018-04-16 /pmc/articles/PMC6021736/ /pubmed/29983966 http://dx.doi.org/10.1002/fsn3.639 Text en © 2018 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Zhu, Suqin
Huang, Min
Feng, Guangxin
Miao, Yu
Wu, Haohao
Zeng, Mingyong
Lo, Yangming Martin
Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
title Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
title_full Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
title_fullStr Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
title_full_unstemmed Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
title_short Gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
title_sort gelatin versus its two major degradation products, prolyl‐hydroxyproline and glycine, as supportive therapy in experimental colitis in mice
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021736/
https://www.ncbi.nlm.nih.gov/pubmed/29983966
http://dx.doi.org/10.1002/fsn3.639
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