Cargando…
The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs
The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxid...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023012/ https://www.ncbi.nlm.nih.gov/pubmed/29614829 http://dx.doi.org/10.3390/bios8020036 |
_version_ | 1783335774927716352 |
---|---|
author | Antunes, Rafael Souza Garcia, Luane Ferreira Somerset, Vernon Sydwill Gil, Eric de Souza Lopes, Flavio Marques |
author_facet | Antunes, Rafael Souza Garcia, Luane Ferreira Somerset, Vernon Sydwill Gil, Eric de Souza Lopes, Flavio Marques |
author_sort | Antunes, Rafael Souza |
collection | PubMed |
description | The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxidation products from such enzymes can be electrochemically reduced, and the sensing is easily achieved by amperometric transducers. In this work, the polyphenoloxidases were extracted from jurubeba (Solanum paniculatum L.) fruits, and the extract was used to construct a carbon paste-based biosensor for pharmaceutical analysis and applications. The assay optimization was performed using a 0.1 mM catechol probe, taking into account the amount of enzymatic extract (50 or 200 μL) and the optimum pH (3.0 to 9.0) as well as some electrochemical differential pulse voltammetric (DPV) parameters (e.g., pulse amplitude, pulse range, pulse width, scan rate). Under optimized conditions, the biosensor was evaluated for the quantitative determination of acetaminophen, acetylsalicylic acid, methyldopa, and ascorbic acid. The best performance was obtained for acetaminophen, which responded linearly in the range between 5 and 245 μM (R = 0.9994), presenting a limit of detection of 3 μM and suitable repeatability ranging between 1.52% and 1.74% relative standard deviation (RSD). |
format | Online Article Text |
id | pubmed-6023012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60230122018-07-02 The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs Antunes, Rafael Souza Garcia, Luane Ferreira Somerset, Vernon Sydwill Gil, Eric de Souza Lopes, Flavio Marques Biosensors (Basel) Article The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxidation products from such enzymes can be electrochemically reduced, and the sensing is easily achieved by amperometric transducers. In this work, the polyphenoloxidases were extracted from jurubeba (Solanum paniculatum L.) fruits, and the extract was used to construct a carbon paste-based biosensor for pharmaceutical analysis and applications. The assay optimization was performed using a 0.1 mM catechol probe, taking into account the amount of enzymatic extract (50 or 200 μL) and the optimum pH (3.0 to 9.0) as well as some electrochemical differential pulse voltammetric (DPV) parameters (e.g., pulse amplitude, pulse range, pulse width, scan rate). Under optimized conditions, the biosensor was evaluated for the quantitative determination of acetaminophen, acetylsalicylic acid, methyldopa, and ascorbic acid. The best performance was obtained for acetaminophen, which responded linearly in the range between 5 and 245 μM (R = 0.9994), presenting a limit of detection of 3 μM and suitable repeatability ranging between 1.52% and 1.74% relative standard deviation (RSD). MDPI 2018-04-02 /pmc/articles/PMC6023012/ /pubmed/29614829 http://dx.doi.org/10.3390/bios8020036 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Antunes, Rafael Souza Garcia, Luane Ferreira Somerset, Vernon Sydwill Gil, Eric de Souza Lopes, Flavio Marques The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs |
title | The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs |
title_full | The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs |
title_fullStr | The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs |
title_full_unstemmed | The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs |
title_short | The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs |
title_sort | use of a polyphenoloxidase biosensor obtained from the fruit of jurubeba (solanum paniculatum l.) in the determination of paracetamol and other phenolic drugs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023012/ https://www.ncbi.nlm.nih.gov/pubmed/29614829 http://dx.doi.org/10.3390/bios8020036 |
work_keys_str_mv | AT antunesrafaelsouza theuseofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT garcialuaneferreira theuseofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT somersetvernonsydwill theuseofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT gilericdesouza theuseofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT lopesflaviomarques theuseofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT antunesrafaelsouza useofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT garcialuaneferreira useofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT somersetvernonsydwill useofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT gilericdesouza useofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs AT lopesflaviomarques useofapolyphenoloxidasebiosensorobtainedfromthefruitofjurubebasolanumpaniculatumlinthedeterminationofparacetamolandotherphenolicdrugs |