FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

A major challenge in single-molecule imaging is tracking the dynamics of proteins or complexes for long periods of time in the dense environments found in living cells. Here, we introduce the concept of using FRET to enhance the photophysical properties of photo-modulatable (PM) fluorophores commonl...

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Detalles Bibliográficos
Autores principales: Basu, Srinjan, Needham, Lisa-Maria, Lando, David, Taylor, Edward J. R., Wohlfahrt, Kai J., Shah, Devina, Boucher, Wayne, Tan, Yi Lei, Bates, Lawrence E., Tkachenko, Olga, Cramard, Julie, Lagerholm, B. Christoffer, Eggeling, Christian, Hendrich, Brian, Klenerman, Dave, Lee, Steven F., Laue, Ernest D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023872/
https://www.ncbi.nlm.nih.gov/pubmed/29955052
http://dx.doi.org/10.1038/s41467-018-04486-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023872/
https://www.ncbi.nlm.nih.gov/pubmed/29955052
http://dx.doi.org/10.1038/s41467-018-04486-0

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