Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain

A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of...

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Autores principales: Han, Xiao, Hou, Lukuan, Hou, Jing, Zhang, Yongyu, Li, Huayue, Li, Wenli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024985/
https://www.ncbi.nlm.nih.gov/pubmed/29865236
http://dx.doi.org/10.3390/md16060191
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author Han, Xiao
Hou, Lukuan
Hou, Jing
Zhang, Yongyu
Li, Huayue
Li, Wenli
author_facet Han, Xiao
Hou, Lukuan
Hou, Jing
Zhang, Yongyu
Li, Huayue
Li, Wenli
author_sort Han, Xiao
collection PubMed
description A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 1–5 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL.
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spelling pubmed-60249852018-07-09 Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain Han, Xiao Hou, Lukuan Hou, Jing Zhang, Yongyu Li, Huayue Li, Wenli Mar Drugs Article A new 14-membered homodimeric macrodiolide, brevidiolide (3), along with four known aromatic compounds (1, 2, 4 and 5) were obtained by heterologous expression of the recombinant plasmid pWLI823 expressing the G231L variant of VioA in the marine-derived Brevibacterium sp. 7002-073. The structures of 1–5 were elucidated on the basis of LC-MS and 2D NMR spectroscopic analyses. In the evaluation for the antibacterial activities of the compounds against multi-drug resistant (MDR) strains, 5 showed notable growth inhibition against Staphylococcus aureus CCARM 3090 and Klebsiella pneumoniae ATCC 13883, with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL. MDPI 2018-06-02 /pmc/articles/PMC6024985/ /pubmed/29865236 http://dx.doi.org/10.3390/md16060191 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Xiao
Hou, Lukuan
Hou, Jing
Zhang, Yongyu
Li, Huayue
Li, Wenli
Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
title Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
title_full Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
title_fullStr Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
title_full_unstemmed Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
title_short Heterologous Expression of a VioA Variant Activates Cryptic Compounds in a Marine-Derived Brevibacterium Strain
title_sort heterologous expression of a vioa variant activates cryptic compounds in a marine-derived brevibacterium strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6024985/
https://www.ncbi.nlm.nih.gov/pubmed/29865236
http://dx.doi.org/10.3390/md16060191
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