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Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins
Sortases are enzymes mostly found in Gram-positive bacteria which cleave proteins site-specifically. This feature makes them a promising tool in molecular biology and biotechnology. In this study, using bacterial surface display of recombinant proteins and ability of sortase A in site-specifically c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026487/ https://www.ncbi.nlm.nih.gov/pubmed/29961206 http://dx.doi.org/10.1186/s13568-018-0638-8 |