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Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins

Sortases are enzymes mostly found in Gram-positive bacteria which cleave proteins site-specifically. This feature makes them a promising tool in molecular biology and biotechnology. In this study, using bacterial surface display of recombinant proteins and ability of sortase A in site-specifically c...

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Detalles Bibliográficos
Autores principales: Fasehee, Hamidreza, Rostami, Amin, Ramezani, Fatemeh, Ahmadian, Gholamreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026487/
https://www.ncbi.nlm.nih.gov/pubmed/29961206
http://dx.doi.org/10.1186/s13568-018-0638-8