Cargando…

Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins

Sortases are enzymes mostly found in Gram-positive bacteria which cleave proteins site-specifically. This feature makes them a promising tool in molecular biology and biotechnology. In this study, using bacterial surface display of recombinant proteins and ability of sortase A in site-specifically c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fasehee, Hamidreza, Rostami, Amin, Ramezani, Fatemeh, Ahmadian, Gholamreza
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2018
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026487/ https://www.ncbi.nlm.nih.gov/pubmed/29961206 http://dx.doi.org/10.1186/s13568-018-0638-8

Ejemplares similares

Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide
por: Rigi, Garshasb, et al.
Publicado: (2021)

Equilibrium Isotherm, Kinetic Modeling, Optimization, and Characterization Studies of Cadmium Adsorption by Surface-Engineered Escherichia coli
por: Tafakori, Vida, et al.
Publicado: (2017)

One-Step Purification of Recombinant Human Amelogenin and Use of Amelogenin as a Fusion Partner
por: Svensson Bonde, Johan, et al.
Publicado: (2012)

Optimization and One-Step Purification of Recombinant V Antigen Production from Yersinia pestis
por: Hosseini, Elahe Seyed, et al.
Publicado: (2020)

Engineering His-Tagged Senecavirus A for One-Step Purification of Viral Antigens
por: Fan, Junhao, et al.
Publicado: (2022)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Optimization of Expression and Purification of Recombinant Mouse plac1
por: Rahdan, Shaghayegh, et al.
Publicado: (2022)

A Robust One-Step Recombineering System for Enterohemorrhagic Escherichia coli
por: Peng, Lang, et al.
Publicado: (2022)

Tag-mediated single-step purification and immobilization of recombinant proteins toward protein-engineered advanced materials
por: Freitas, Ana I., et al.
Publicado: (2021)

One Step Purification—Vaccine Delivery System
por: Soto, Ernesto R., et al.
Publicado: (2023)

Expression and purification of an influenza hemagglutinin—one step closer to a recombinant protein-based influenza vaccine
por: Wang, Keyang, et al.
Publicado: (2006)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli
por: Seetaraman Amritha, Trikkur Madom, et al.
Publicado: (2020)

Pore Engineering for One-Step Ethylene Purification from a Three-Component Hydrocarbon Mixture
por: Zhu, Baoyong, et al.
Publicado: (2021)

High level expression and purification of recombinant flounder growth hormone in E. coli
por: Choi, Tae-Jin, et al.
Publicado: (2018)

Cloning, expression and purification of Chikungunya virus E2 recombinant protein in E.coli
por: Kr Verma, Anil, et al.
Publicado: (2014)

Expression and purification of functional recombinant CUL2•RBX1 from E. coli
por: Diaz, Stephanie, et al.
Publicado: (2021)

Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural Characterisation
por: Loch, Joanna I., et al.
Publicado: (2016)

A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli
por: Ederth, Josefine, et al.
Publicado: (2009)

Effects of Process Parameters on the Characteristics of Mixed-Halide Perovskite Solar Cells Fabricated by One-Step and Two-Step Sequential Coating
por: Ahmadian-Yazdi, Mohammad Reza, et al.
Publicado: (2016)

A positive Selection Escherichia Coli Recombinant Protein Expression Vector for One-Step Cloning
por: James, Shinto, et al.
Publicado: (2022)

Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
por: Shankar, Ram, et al.
Publicado: (2021)

Rapid one-step recombinational cloning
por: Fu, Changlin, et al.
Publicado: (2008)

Improvement of solubility and yield of recombinant protein expression in E. coli using a two-step system
por: Sadeghian-Rizi, Tahereh, et al.
Publicado: (2019)

Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli
por: Malik, Ajamaluddin
Publicado: (2016)

Complete Solubilization and Purification of Recombinant Human Growth Hormone Produced in Escherichia coli
por: Kim, Min-Ji, et al.
Publicado: (2013)

Optimization of the recombinant production and purification of a self-assembling peptide in Escherichia coli
por: Rad-Malekshahi, Mazda, et al.
Publicado: (2014)

Expression optimization of recombinant cholesterol oxidase in Escherichia coli and its purification and characterization
por: Fazaeli, Aliakbar, et al.
Publicado: (2018)

Production and Easy One-Step Purification of Bluetongue Recombinant VP7 from Infected Sf9 Supernatant for an Immunoenzymatic Assay (ELISA)
por: Ulisse, S., et al.
Publicado: (2020)

Refolding and purification of recombinant L-asparaginase from inclusion bodies of E. coli into active tetrameric protein
por: Upadhyay, Arun K., et al.
Publicado: (2014)

Expression and purification of LipL32 recombinant protein of Leptospira interrogans in different E.coli strains
por: Bastos, Victor, et al.
Publicado: (2014)

One-step purification and characterization of alginate lyase from a clinical Pseudomonas aeruginosa with destructive activity on bacterial biofilm
por: Ghadam, Parinaz, et al.
Publicado: (2017)

Expression and Single-step Purification of GRA8 Antigen of Toxoplasma gondii in Escherichia coli
por: Babaie, Jalal, et al.
Publicado: (2011)

Engineering formation of multiple recombinant Eut protein nanocompartments in E. coli
por: Held, Mark, et al.
Publicado: (2016)

Cloning, Optimization of Periplasmic Expression and Purification of Recombinant Granulocyte Macrophage-Stimulating Factor in Escherichia coli BL21 (DE3)
por: Taherian, Elham, et al.
Publicado: (2019)

One-step purification of assembly-competent tubulin from diverse eukaryotic sources
por: Widlund, Per O., et al.
Publicado: (2012)

Novel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins
por: Jiang, Min, et al.
Publicado: (2017)

One-Step Purification of Microbially Produced Hydrophobic Terpenes via Process Chromatography
por: Grozdev, Ljubomir, et al.
Publicado: (2019)

One-Step Versatile Fabrication of Superhydrophilic Filters for the Efficient Purification of Oily Water
por: Kim, Seongmin, et al.
Publicado: (2021)

Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli
por: Das, Krishna M.P., et al.
Publicado: (2011)

High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG
por: Luo, Dan, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_kfe7ic20mn10cl9moj0jlc38qm