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Effect of sequential C‐terminal tryptophans on green fluorescent protein fluorescence

The effect of the addition of sequential C‐terminal tryptophan residues on the fluorescence intensity of GFP was investigated. Tandem repeats of six tryptophan residues markedly decreased fluorescence intensity. This phenomenon is likely to occur because of the inhibition of GFP folding, resulting i...

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Detalles Bibliográficos
Autores principales: Tsuchida, Shirou, Kanashiki, Takumi, Izumiya, Shuhei, Ichikawa, Takuya, Kurosawa, Ryusuke, Hamaue, Naoya, Aoki, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026694/
https://www.ncbi.nlm.nih.gov/pubmed/29988552
http://dx.doi.org/10.1002/2211-5463.12445