Cargando…

Effect of sequential C‐terminal tryptophans on green fluorescent protein fluorescence

The effect of the addition of sequential C‐terminal tryptophan residues on the fluorescence intensity of GFP was investigated. Tandem repeats of six tryptophan residues markedly decreased fluorescence intensity. This phenomenon is likely to occur because of the inhibition of GFP folding, resulting i...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tsuchida, Shirou, Kanashiki, Takumi, Izumiya, Shuhei, Ichikawa, Takuya, Kurosawa, Ryusuke, Hamaue, Naoya, Aoki, Takashi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2018
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026694/ https://www.ncbi.nlm.nih.gov/pubmed/29988552 http://dx.doi.org/10.1002/2211-5463.12445

Ejemplares similares

Effects of naturally occurring missense mutations and G525V in the hydratase domain of human d-bifunctional protein on hydratase activity
por: Tsuchida, Shirou, et al.
Publicado: (2014)

Protein–protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy
por: Oyama, Rieko, et al.
Publicado: (2006)

Development of a method for screening short-lived proteins using green fluorescent protein
por: Jiang, Xin, et al.
Publicado: (2004)

Reassembling green fluorescent protein for in vitro evaluation of trans-translation
por: Guyomar, Charlotte, et al.
Publicado: (2020)

RNA aptamers that functionally interact with green fluorescent protein and its derivatives
por: Shui, Bo, et al.
Publicado: (2012)

Live-cell imaging of single mRNA dynamics using split superfolder green fluorescent proteins with minimal background
por: Park, Sung Young, et al.
Publicado: (2020)

Near-infrared fluorescent northern blot
por: Miller, Bret R., et al.
Publicado: (2018)

BENA435, a new cell-permeant photoactivated green fluorescent DNA probe
por: Erve, Alexandra, et al.
Publicado: (2006)

Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences
por: Ali, Rizwan, et al.
Publicado: (2018)

Isolation, Purification, and Culture of Embryonic Melanoblasts from Green Fluorescent Protein–expressing Reporter Mice
por: Crawford, Melissa, et al.
Publicado: (2023)

A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis(†)
por: Turcatti, Gerardo, et al.
Publicado: (2008)

Improved fluorescent signal in expansion microscopy using fluorescent Fab fragment secondary antibodies
por: Sherry, David M., et al.
Publicado: (2022)

Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy
por: Walsh, Matthew T., et al.
Publicado: (2017)

Usefulness of blood supply visualization by indocyanine green fluorescence for reconstruction during esophagectomy
por: Shimada, Yutaka, et al.
Publicado: (2011)

A dual fluorescent reporter for the investigation of methionine mistranslation in live cells
por: Gomes, Ana Cristina, et al.
Publicado: (2016)

Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming
por: Schmitt, Christopher E., et al.
Publicado: (2017)

Tryptophan-based chromophore in fluorescent proteins can be anionic
por: Sarkisyan, Karen S., et al.
Publicado: (2012)

Molecular beacons with intrinsically fluorescent nucleotides
por: Martí, Angel A., et al.
Publicado: (2006)

Fluorescent signatures for variable DNA sequences
por: Rice, John E., et al.
Publicado: (2012)

Improved mini-Tn7 Delivery Plasmids for Fluorescent Labeling of Stenotrophomonas maltophilia
por: Mamat, Uwe, et al.
Publicado: (2023)

Tryptophan and Non-Tryptophan Fluorescence of the Eye Lens Proteins Provides Diagnostics of Cataract at the Molecular Level
por: Gakamsky, Anna, et al.
Publicado: (2017)

Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi
por: Takacs, Constantin N., et al.
Publicado: (2018)

Single-Cell Resolution of Uncultured Magnetotactic Bacteria via Fluorescence-Coupled Electron Microscopy
por: Li, Jinhua, et al.
Publicado: (2017)

A simple, rapid, and sensitive fluorescence-based method to assess triacylglycerol hydrolase activity
por: Rajan, Sujith, et al.
Publicado: (2021)

Fluorescent Protein Expression as a Proxy for Bacterial Fitness in a High-Throughput Assay
por: Schlechter, Rudolf O., et al.
Publicado: (2021)

Application of the Fluorescence-Activating and Absorption-Shifting Tag (FAST) for Flow Cytometry in Methanogenic Archaea
por: Adlung, Norman, et al.
Publicado: (2023)

Mechanism of the Efficient Tryptophan Fluorescence Quenching in Human γD-Crystallin Studied by Time-Resolved Fluorescence
por: Chen, Jiejin, et al.
Publicado: (2008)

Fluorescence-based characterization of non-fluorescent transient states of tryptophan – prospects for protein conformation and interaction studies
por: Hevekerl, Heike, et al.
Publicado: (2016)

Postmortem fluorescence angiography of the explanted human heart
por: Lux, Constantin, et al.
Publicado: (2021)

Fluorescent Biosensor Imaging of Nitrate in Arabidopsis thaliana
por: Chen, Yen-Ning, et al.
Publicado: (2023)

Determination of epristeride by its quenching effect on the fluorescence of L-tryptophan
por: Gong, Ai-Qin, et al.
Publicado: (2013)

A New Mouse Line Reporting the Translation of Brain-Derived Neurotrophic Factor Using Green Fluorescent Protein
por: Wosnitzka, Erin, et al.
Publicado: (2020)

MiL-FISH: Multilabeled Oligonucleotides for Fluorescence In Situ Hybridization Improve Visualization of Bacterial Cells
por: Schimak, Mario P., et al.
Publicado: (2015)

Feasibility of Bone Perfusion Evaluation in Cadavers Using Indocyanine Green Fluorescence Angiography
por: Yoshimatsu, Hidehiko, et al.
Publicado: (2017)

Mix and measure fluorescence screening for selective quadruplex binders
por: Paramasivan, Sattanathan, et al.
Publicado: (2008)

Rapid DNA mapping by fluorescent single molecule detection
por: Xiao, Ming, et al.
Publicado: (2007)

Fluorescent T7 display phages obtained by translational frameshift
por: Slootweg, Erik J., et al.
Publicado: (2006)

Fluorescence strategies for high-throughput quantification of protein interactions
por: Hieb, Aaron R., et al.
Publicado: (2012)

Construction of repeat-free fluorescence in situ hybridization probes
por: Swennenhuis, Joost F., et al.
Publicado: (2012)

Fluorescence spectroscopic detection and measurement of single telomere molecules
por: Beh, Cyrus W, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_t62jb58c2n4rcn4vnmfl7sigj1