Efficient CRISPR‐based genome editing using tandem guide RNAs and editable surrogate reporters

Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat (CRISPR)‐based gene editing, particularly when the given guide RNA exhibits low cleavage activity. Here, we describe the packaging of tandem guide RNAs and single‐strand annealing‐based surrogate reporte...

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Detalles Bibliográficos
Autores principales: Liu, Wuqing, Li, Shifeng, Zhang, Yunbin, Li, Jinsong, Li, Yiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026697/
https://www.ncbi.nlm.nih.gov/pubmed/29988563
http://dx.doi.org/10.1002/2211-5463.12437

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026697/
https://www.ncbi.nlm.nih.gov/pubmed/29988563
http://dx.doi.org/10.1002/2211-5463.12437

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