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Efficient CRISPR‐based genome editing using tandem guide RNAs and editable surrogate reporters
Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat (CRISPR)‐based gene editing, particularly when the given guide RNA exhibits low cleavage activity. Here, we describe the packaging of tandem guide RNAs and single‐strand annealing‐based surrogate reporte...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026697/ https://www.ncbi.nlm.nih.gov/pubmed/29988563 http://dx.doi.org/10.1002/2211-5463.12437 |