Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher

[Image: see text] The near-infrared fluorescent activatable smart probe Pyro-phosphatidylethanolamine (PtdEtn)-QSY was synthesized and observed to selectively fluoresce in the presence of phosphatidylcholine-specific phospholipase C (PC-PLC). PC-PLC is an important biological target as it is known t...

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Autores principales: Liebov, Benjamin K., Arroyo, Alejandro D., Rubtsova, Natalia I., Osharovich, Sofya A., Delikatny, E. James, Popov, Anatoliy V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026834/
https://www.ncbi.nlm.nih.gov/pubmed/29978148
http://dx.doi.org/10.1021/acsomega.8b00635
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author Liebov, Benjamin K.
Arroyo, Alejandro D.
Rubtsova, Natalia I.
Osharovich, Sofya A.
Delikatny, E. James
Popov, Anatoliy V.
author_facet Liebov, Benjamin K.
Arroyo, Alejandro D.
Rubtsova, Natalia I.
Osharovich, Sofya A.
Delikatny, E. James
Popov, Anatoliy V.
author_sort Liebov, Benjamin K.
collection PubMed
description [Image: see text] The near-infrared fluorescent activatable smart probe Pyro-phosphatidylethanolamine (PtdEtn)-QSY was synthesized and observed to selectively fluoresce in the presence of phosphatidylcholine-specific phospholipase C (PC-PLC). PC-PLC is an important biological target as it is known to be upregulated in a variety of cancers, including triple negative breast cancer. Pyro-PtdEtn-QSY features a QSY21 quenching moiety instead of the Black Hole Quencher-3 (BHQ-3) used previously because the latter contains an azo bond, which could lead to biological instability.
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spelling pubmed-60268342018-07-03 Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher Liebov, Benjamin K. Arroyo, Alejandro D. Rubtsova, Natalia I. Osharovich, Sofya A. Delikatny, E. James Popov, Anatoliy V. ACS Omega [Image: see text] The near-infrared fluorescent activatable smart probe Pyro-phosphatidylethanolamine (PtdEtn)-QSY was synthesized and observed to selectively fluoresce in the presence of phosphatidylcholine-specific phospholipase C (PC-PLC). PC-PLC is an important biological target as it is known to be upregulated in a variety of cancers, including triple negative breast cancer. Pyro-PtdEtn-QSY features a QSY21 quenching moiety instead of the Black Hole Quencher-3 (BHQ-3) used previously because the latter contains an azo bond, which could lead to biological instability. American Chemical Society 2018-06-25 /pmc/articles/PMC6026834/ /pubmed/29978148 http://dx.doi.org/10.1021/acsomega.8b00635 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Liebov, Benjamin K.
Arroyo, Alejandro D.
Rubtsova, Natalia I.
Osharovich, Sofya A.
Delikatny, E. James
Popov, Anatoliy V.
Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher
title Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher
title_full Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher
title_fullStr Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher
title_full_unstemmed Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher
title_short Nonprotecting Group Synthesis of a Phospholipase C Activatable Probe with an Azo-Free Quencher
title_sort nonprotecting group synthesis of a phospholipase c activatable probe with an azo-free quencher
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6026834/
https://www.ncbi.nlm.nih.gov/pubmed/29978148
http://dx.doi.org/10.1021/acsomega.8b00635
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