Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene

During CRISPR/Cas9 mediated genome editing, site-specific double strand breaks are introduced and repaired either unspecific by non-homologous end joining (NHEJ) or sequence dependent by homology directed repair (HDR). Whereas NHEJ-based generation of gene knock-out is widely performed, the HDR-base...

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Detalles Bibliográficos
Autores principales: Gerlach, Max, Kraft, Theresia, Brenner, Bernhard, Petersen, Björn, Niemann, Heiner, Montag, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027509/
https://www.ncbi.nlm.nih.gov/pubmed/29899280
http://dx.doi.org/10.3390/genes9060296

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027509/
https://www.ncbi.nlm.nih.gov/pubmed/29899280
http://dx.doi.org/10.3390/genes9060296

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