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Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene

During CRISPR/Cas9 mediated genome editing, site-specific double strand breaks are introduced and repaired either unspecific by non-homologous end joining (NHEJ) or sequence dependent by homology directed repair (HDR). Whereas NHEJ-based generation of gene knock-out is widely performed, the HDR-base...

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Detalles Bibliográficos
Autores principales:	Gerlach, Max, Kraft, Theresia, Brenner, Bernhard, Petersen, Björn, Niemann, Heiner, Montag, Judith
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6027509/ https://www.ncbi.nlm.nih.gov/pubmed/29899280 http://dx.doi.org/10.3390/genes9060296

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