Tailoring recombinant lipases: keeping the His-tag favors esterification reactions, removing it favors hydrolysis reactions

We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus (TEV) protease. Circular dichroism analysis sh...

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Detalles Bibliográficos
Autores principales: de Almeida, Janaina Marques, Moure, Vivian Rotuno, Müller-Santos, Marcelo, de Souza, Emanuel Maltempi, Pedrosa, Fábio Oliveira, Mitchell, David Alexander, Krieger, Nadia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030132/
https://www.ncbi.nlm.nih.gov/pubmed/29968752
http://dx.doi.org/10.1038/s41598-018-27579-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030132/
https://www.ncbi.nlm.nih.gov/pubmed/29968752
http://dx.doi.org/10.1038/s41598-018-27579-8

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