Cargando…
Tailoring recombinant lipases: keeping the His-tag favors esterification reactions, removing it favors hydrolysis reactions
We determined the effect of the His-tag on the structure, activity, stability and immobilization of LipC12, a highly active lipase from a metagenomic library. We purified LipC12 with a N-terminal His-tag and then removed the tag using tobacco etch virus (TEV) protease. Circular dichroism analysis sh...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030132/ https://www.ncbi.nlm.nih.gov/pubmed/29968752 http://dx.doi.org/10.1038/s41598-018-27579-8 |